Source: ADA TECHNOLOGIES, INC. submitted to
CHEMICALLY ACTIVE FRUIT LABEL FOR THE VISUAL ESTIMATION OF FRUIT RIPENESS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0197094
Grant No.
2003-33610-13943
Project No.
COLK-2003-04100
Proposal No.
2003-04100
Multistate No.
(N/A)
Program Code
8.9
Project Start Date
Sep 1, 2003
Project End Date
Dec 31, 2006
Grant Year
2003
Project Director
Lovell, J. S.
Recipient Organization
ADA TECHNOLOGIES, INC.
8100 SHAFFER PKWY., SUITE 130
LITTLETON,CO 80127
Performing Department
(N/A)
Non Technical Summary
Certain fruits do not change color as they ripen and hence it may be difficult for consumers to select fruit that is ready to eat. Melons and winter pears are examples of fruit that is difficult to select. Most fruit is now individually labeled and this project will develop a label that will change color as the fruit approaches ripeness. This colorimetric label will provide the consumer with an immediate visual clue that will allow them to select fruit that is approaching ripeness, or is ripe. The label will be inherently safe, in case of accidental ingestion and the incremental cost of the label over the current labels will be small.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
5031115100030%
5031115102020%
5031115200050%
Goals / Objectives
The skin color of some fruits does not change color as the fruit ripens. Examples of these fruits include winter pears, melons and kiwi fruit. This makes it difficult for unsophisticated consumers to select fruit, with the result that they may have an unsatisfactory experience with the fruit and will, therefore be reluctant to repeat it. The objective of this project will be to develop a colorimetric label that will respond to the ripening process of fruit. The label will be designed to respond to the increasing flux of ethylene as the fruit approached optimal ripeness. The color change will provide the consumer with an immediate visual clue that will allow them to select fruit that is in an ideal condition for eating, either immediately or later. If the consumers have a positive experience when they eat fruit in optimal condition they will be more inclined to repeat it, thus leading to an increasing demand for the fruit and higher prices to the growers. The principal objectives will be to develop a label that will be sufficiently sensitive to show an incremental color change over a period of several days, as the fruit ripens. Because of the risks of accidental ingestion, the reagents must be inherently safe and must be present in quantities that do not offer any risk to the consumer. The labels will be designed to be compatible with current labeling machinery and the incremental cost must be low to achieve acceptance from the growers.
Project Methods
Ripening of fruit involves a number of changes. In fleshy fruit, the chlorophyll is degraded, and other pigments may form, changing the fruit color. Simultaneously, the fleshy part of the fruit softens, due to the enzymatic digestion of pectin, the principal component of the middle lamellae. As the middle lamellae are weakened, cells are able to slip past each other. During the same period, starches and organic acids, or, in the case of avocados, oils, are metabolized into sugars. During the time of ripening of certain fruits such as pears, melons and some tropical fruits, there is a large increase in cellular respiration, evidenced by increased uptake of oxygen and the evolution of ethylene. This phase is known as the climacteric. After the climacteric, senescence sets in and the fruit becomes susceptible to fungi and other microorganisms. Ethylene production actually begins before the climacteric but reaches a maximum at the climacteric. Once the climacteric has passed the production of ethylene falls precipitously. Climacteric fruit include winter pears, avocados, kiwi fruit, melons and certain tropical fruits. Citrus fruit and apples do not pass through a climacteric phase. There have been a number of test methods developed to determine the ripeness of fruit. Test methods have included spectrometric measurements of fruit colors (tomatoes, apples, peaches, apricots), optical density (pears), nuclear magnetic resonance (nectarines, oranges, cherries), reflectance (bananas, apples), gas-sensing and odor (apples, bananas, papaya, melons), and impact (avocados, melons). Many of these techniques were intended to assist in assessing the harvest maturity and/or sorting in the packing house and are not for use by the consumers at the point of purchase. Most produce is now labeled. The labels carry the Price Look-Up number to identify the variety and other information such as the producer or packer. This project will exploit the production of ethylene as an indicator of fruit ripeness by developing a modification to existing labels that will respond to the cumulative evolution of ethylene by a colorimetric reaction with the reagents in the label. The color of the label will provide immediate feedback to consumers, allowing them to select fruit that is unripe, close to ripeness, or ready to eat. The label will be compatible with current labeling machinery and materials and will safe for consumers. The label design will include multiple layers to isolate the colorimetric reagents from the skin of the fruit and to contain all of the ethylene within the reactive layer. The label will be designed to be compatible with current labeling machinery and materials and costs will be of the order of 0.1c per label for pears.

Progress 09/01/03 to 12/31/06

Outputs
The original objective of this SBIR project was to develop a label that could be printed, assembled and applied to fruit using existing equipment. The goal was to label every piece of fruit individually. A label was developed using a three layer system and a colorimetric indicator that has shown reliably to indicate that the fruit had reached the climacteric and an optimal state of ripeness. In the course of the project, we learned that the evolution rate of different pear cultivars such as Bartlett, Comice and Bosc, evolve ethylene at different rates to d'Anjou pears. Furthermore, we learned that the storage times of d'Anjou pears can influence the time taken to reach the climacteric and affects the amount of ethylene released during the process. Nectarines and melons did not appear to release measurable amounts of ethylene and there was no indication of a climacteric. During the project, ADA developed a colorimetric reaction that reacts to hydrogen iodide producing a change in pH that can be followed with a pH indicator. This reaction appears to be similar, if not identical to a system that has in parallel been commercialized by Diamond Fruit Growers and a New Zealand company, HortResearch. ADA submitted an application for a patent on this technology in September 2004. It seems likely that HortResearch has also applied for a patent, but, to date, there is no reference to either patent from the US Patent and Trademark Office. Which company will receive the patent rights still remains to be seen. Our putative commercial partner did not feel that the three layer label can be applied to fruit in a reliable manner at typical labeling volumes. The labels used in the experiments were painstakingly cut from the polymers and the filter paper and applied by hand. It was not considered possible to convert this to an automated process for high speed (i.e. low cost) label manufacture and application; however this does not mean that the concept is of no commercial value. While it has unfortunately been concluded that this investigation was not able to lead to a low-cost, high-volume, reliable, printable label, it may be possible to continue to explore volume manufacturing of the three-layer concept. Diamond Fruit / HortResearch appear to have developed a method for fabricating the three layer label, but not for individual pear application. Thus it is encouraging that it may be possible to use a less high speed process than that used by Sinclair to make the labels for application directly to fruit at a cost that would be less than the blister pack used with the HortResearch package.

Impacts
Certain fruits can be difficult for consumers to select, with the result that they may have an unsatisfactory experience which they will be reluctant to repeat. The consumer will be able to tell, at a glance, whether the fruit is not ready to eat or is near-ripe, ripe, or over-ripe. If the consumer is guaranteed a pleasurable experience, the grower should benefit by increased demand.

Publications

  • No publications reported this period