Progress 10/01/04 to 09/30/09
Outputs
Progress Report Objectives (from AD-416) 1) Evaluate cold tolerance of winter wheat recombinant inbred and near isogenic wheat populations by quantifying cold tolerance per se, and vernalization response. 2) Identify QTL's associated with those traits and develop molecular markers for use in marker-assisted selection for cold tolerance. 3) Fine map the frost resistance gene (Fr2) on chromosome 5A in diploid Triticum monococcum. 4) Characterize the CBF gene family indiploid wheat, determine which CBF genes are linked to Fr2, and compare locations of CBF genes with locations of QTL's for cold tolerance identified above. Approach (from AD-416) Methods used include artificial freeze tests, determination of flowering response under increasing daylight regines, linkage analysis using microsatellites, AFLPs, RFLPs, SNPs and candidate gene sequeces, analysis of diploid wheat BAC libraries. Statistical methods used include analysis of variance and maximum likelihood estimation of linkage. Results will provide genetic resources for mapping and cloning cold tolerance genes in wheat and more efficient methods of selection for cold tolerance. Results will also be applicable to other crops (for example winter barley). Documents Reimbursable with NRI. Log 25968. Formerly 5348- 21220-003-06R (5/2008). Significant Activities that Support Special Target Populations Genetic factors conferring cold tolerance in wheat. The question was where were the major loci influencing cold acclimation and resistance to freezing in wheat. We assayed wheat tolerance to cold in artificial freezing tests, mapped loci associated with tolerance, and created fine mapping populations to better isolate genes conferring cold tolerance. We identified 5 loci that are associated with resistance to freezing in two winter wehat populations one of which is a major locus, Fr2 on chromosome 5L. The Fr2 locus may function as a major regulator of cold tolerance in wheat which would result in breeders being able to improve winter survival. This research is relevant to NP301 Component 2 �Crop Informatics, Genomics, and Genetic Analyses� and to National Program Problem Statements NP301 2C: Genetic Analyses and Mapping of Important Traits, and NP302 2B: Understanding Plant Interactions with Their Environment.
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) 1) Evaluate cold tolerance of winter wheat recombinant inbred and near isogenic wheat populations by quantifying cold tolerance per se, and vernalization response. 2) Identify QTL's associated with those traits and develop molecular markers for use in marker-assisted selection for cold tolerance. 3) Fine map the frost resistance gene (Fr2) on chromosome 5A in diploid Triticum monococcum. 4) Characterize the CBF gene family indiploid wheat, determine which CBF genes are linked to Fr2, and compare locations of CBF genes with locations of QTL's for cold tolerance identified above. Approach (from AD-416) Methods used include artificial freeze tests, determination of flowering response under increasing daylight regines, linkage analysis using microsatellites, AFLPs, RFLPs, SNPs and candidate gene sequeces, analysis of diploid wheat BAC libraries. Statistical methods used include analysis of variance and maximum likelihood estimation of linkage. Results will provide genetic resources for mapping and cloning cold tolerance genes in wheat and more efficient methods of selection for cold tolerance. Results will also be applicable to other crops (for example winter barley). Documents Reimbursable with NRI. Log 25968. Significant Activities that Support Special Target Populations This report serves to document research conducted under a reimbursable agreement between USDA-CSREES-NRI and the USDA-ARS. Additional details of research can be found in the report for parent CRIS PROJECT NUMBER: 5348-21220-003-00D, IDENTIFICATION AND DEPLOYMENT OF GENES TO REDUCE PRODUCTION RISKS AND IMPROVE QUALITY IN CLUB AND SOFT WHITE WINTER WHEAT. Quantitative trait loci (QTLs) for cold tolerance and vernalization requirement have been identified in two winter wheat populations, Norstar/Centurk78 and Karl/Z0031 on chromosome 5A that overlap the region containing CBF gene homologues that are associated with cold tolerance in other species. The problem being addressed is genetic control of winter survival in winter wheat. This project is being accomplished by Dr. Latha Reddy who is working in Dr. Garland Campbell�s laboratory. Results of this research will improve efforts to reduce grower risk of winter injury in wheat and the QTLs will be used to select for more cold tolerant winter wheat. Progress on the collaborative project is monitored through monthly meetings with other scientists and through emails containing data and photographs.
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Progress 10/01/05 to 09/30/06
Outputs
Progress Report 4d Progress report. This report serves to document research conducted under a reimbursable agreement between ARS and NRI-CGP, CSREES. The objectives were in support of the long-term goal of the parent CRIS, 5348-21220-003-00D, Identification and Deployment of Genes to Reduce Production Risks and Improve Quality in Club and Soft White Winter Wheat. Quantitative trait loci (QTLs) for cold tolerance and vernalization requirement have been identified in two winter wheat populations, Norstar/Centurk78 and Karl/Z0031. QTLs on chromosomes 5A have been identified in both populations that overlap the region containing CBF gene homologues that are associated with cold tolerance in other species. The problem being addressed is identification of genetic factors conferring winter hardiness in winter wheat. The work is being accomplished by one WSU graduate student in Dr. Garland Campbell's laboratory. The QTLs will be used to select for more cold tolerant winter
wheat.
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Progress 10/01/04 to 09/30/05
Outputs
4d Progress report. This report serves to document research under a reimbursable agreement between ARS and the USDA-CSREES-NRI Plant Genome program. This research is conducted in collaboration with Dr. J. Dubcovsky of University of California at Davis, CA. Additional details my be found in the report of the parent project 5348-21000-019-00D. We are using simple sequence repeat (SSR) markers to identify quantitative trait loci (QTLs) for cold tolerance and vernalization requirement, and to identify markers that can be used for marker assisted selection for winter hardiness. Two recombinant inbred line(RIL) populations, Norstar/Centurk78 and Karl/Z0031, are being used to map QTLs for cold tolerance. Initial screening has been done using SSR primers of the gwm and barc series, 119 primers are polymorphic for the Norstar/Centurk78 population and 137 polymorphic primers are identified for the Karl/Z0031 population. These two populations have been replicated and tested
for their cold hardiness in the greenhouse using an artificial freeze test. Their LT50 values (the temperature at which 50% of the plants of a given genotype survive after freezing) have been determined. The parental LT50 values ranged from 12.43oC for Z0031, 18.19oC for Karl, -18.62oC for Norstar and -16.01oC for Centurk78 respectively. The range of population values for the Norstar/Centurk78 population exceeds that of the parents. One putative QTL has been identified in the Norstar/Centurk population near gwm518 on chromosome 6BS. Two QTLs have been identified in the Karl/Z0031 population, one may be on chromosome 4B associated with the markers gwm368 and gwm165. The other may be on chromosome 5B associated with the markers gwm67 and gwm133 (the location of gwm133 has not been verified).
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