Source: AGRICULTURAL RESEARCH SERVICE submitted to
MEDITERRANEAN FRUIT FLY GENOME SEQUENCING
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
NEW
Funding Source
Reporting Frequency
Annual
Accession No.
0412299
Grant No.
(N/A)
Project No.
0206-22000-001-20R
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Jul 1, 2008
Project End Date
Sep 30, 2008
Grant Year
(N/A)
Project Director
VICK K W
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
USDA-ARS-ANRI-BFGL
BELTSVILLE,MD 20705
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
(N/A)
Research Effort Categories
Basic
100%
Applied
0%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
99024999900100%
Knowledge Area
990 - Unclassified;

Subject Of Investigation
2499 - Plant research, general;

Field Of Science
9900 - Unclassified;
Goals / Objectives
The objective of this cooperative research project is to sequence the genome of the Mediterannean fruit fly, Ceratitis capitata, and to assemble these sequences so that subsequent annotation and functional analysis of the genomic sequences may be acheived. This project will also provide the basis for sequencing other important tephritid pest species.
Project Methods
Sequencing will be performed using the 454 XLR-high density pyrosequencing system. The initial phase of the project will support sequencing and sequence assembly of approximately seven full-chip production runs yielding approximately 2,800 million nucleotide sequences, representing 40% of a complete genome sequence project (assuming a final goal of a 12-fold sequencing coverage of the 540Mb genome). Genomic sequence assembly will be acheived by computational analyses.