Progress 09/15/05 to 03/14/10

Outputs
OUTPUTS: Activities: For genetic improvement of yellow perch, eight geographical strains of yellow perch broodfish were obtained from different states. Differentiation among the populations was quantified by analysis of genetic differentiation. Two round of selection were completed from selected broodfish of 2005 year-class. For bluegill, about 15,000 all-male and 5,000 super male populations were reared to maturation for developing all-male broodstock. A first linkage map of bluegill was constructed for male and female for detection of important commercial traits in the future. We completed a project "Establishment of mostly-male groups of bluegill by size-selection and evaluation of their growth performance". For the Bowling Green Aquaculture Program, preliminary trials with on-farm cooperators has shown that another baitfish species, the golden shiner, can be successfully raised to market size in one growing season. Preliminary pond trials with spotfin shiners at a cooperator's ponds demonstrated they can be successfully grown to market size (2 in) in one growing season. A workshop was held to teach the basics of system selection, management and potential of recirculating aquaculture systems in the north central region. Our electrophoretic proteomic methodology concentrated on developing a novel high throughput functional proteomic analysis to identify muscle proteins associated with body weight and length in yellow perch. Project "Proteomic analysis of whole muscle from yellow perch: Identification of proteins associated with body mass and length" was completed. An assessment of the Ohio State University Aquaculture Program (OSUAP) was completed. Information is being used to determine stakeholder needs for the future. The OARDIP outreach activities encompass a variety of methods to educate producers on all aspects of profitably producing a sustainable aquaculture product. Events: Multiple annual workshops and presentations are conducted to address stakeholder needs, as well as individual mentoring and training and 24/7 web support (southcenters.osu.edu/aqua). Services: Site visits, personal and phone consulting regarding all aspects of freshwater fish and shrimp culture in Ohio are available during business hours. At the Bowling Green Aquaculture Center, additional technical support was provided throughout the growing season through site visits and weekly phone calls. Products: Two groups of the 2nd generation of improved fish have been created. A linkage map of bluegill was constructed. A webpage (http://www.ag.ohio-state.edu/~prec/aqua/shrimp.htm) with links to freshwater shrimp information has been created and is available to clients 24/7. PARTICIPANTS: Ottobre, J. S. Director of project. Overall coordination. Wang, H. P., Director of GIFT (Genetic Improvement of Farmed-fish Traits) Program. Wallat, G,. Director of Bowling Green Aquaculture Program. Tiu, L. G., Director of Aquaculture Technology Program. Wick, M., Director of Fish Muscle Growth and Nutrition Program. TARGET AUDIENCES: Target audiences are aquacultural producers in Ohio. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The selected generations of superior broodfish consolidated the foundation for breeding programs for yellow perch and bluegill. Forty-two microsatellite markers were developed and eight of them were optimized for parentage analysis for the breeding program. The improved growth rate of 25-54% of selected yellow perch lines vs. controls shows a great premise of the breeding program and potential impact on the yellow perch industry. The success of sex-reversing regular fish to all females was published in Aquaculture. A first linkage map of bluegill was published in Aquaculture International (2009) and laid a foundation for detection of important commercial traits in the future for the bluegill. A practical strategy for establishing mostly-male bluegill groups that can gain 61.0% more weight than the mixed-sex control was published in North American Journal of Aquaculture (2009). Bowling Green research site provides a physical demonstration facility of RAS technology for clientele in the northwest Ohio region, as well as the personnel with experience in RAS to provide outreach training and consultation. Preliminary investigations showed that market-size spotfin shiners and golden shiners can be raised in one growing season in Ohio's temperate climate. This success has spurred interest from other growers, looking to expand or develop new aquaculture markets in Ohio. A new commercial-scale golden shiner incubation system was developed, improving hatch out and swim up survival to 75%. The paper "Proteomic analysis of whole muscle from yellow perch, Perca flavescens: Identification of proteins associated with body mass and length" was published in Proteomics. In 2005, only 24 farms reported 29 full-time employees and 46 part-time employees. The entire project has had import impacts on Ohio aquaculture through research and technology transfer. Aquaculture sales in Ohio have tripled from $1.8 million to $6.6 million in recent years. In 2010, 66 farms reported 75 full-time employees and 81 part-time employees in Ohio. In 2005 the North Central Region of the U.S., Ohio ranks 3rd among the 12 states in the region for total sales of aquaculture products, up from 7th place in 1998. Nationally, Ohio ranks first in sales of yellow perch for food and is the number one bluegill producing state. Ohio also ranks fourth in sales of baitfish and largemouth bass sold for sport and fifth in number of baitfish farms. Thirteen journal articles and proceedings papers were published.

Publications

• Wang, H.P. Z. Gao, B. Beres, J. Ottobre, G.K. Wallat, L.G. Tiu, D. Rapp, P. OBryant and H. Yao. 2008. Effects of estradiol-17 on survival, growth performance, sex reversal and gonadal structure of bluegill sunfish Lepomis macrochirus. Aquaculture 285: 216-223
• Wang, H.P., G.K. Wallat, P. OBryant, J. D. Rapp, H. Yao, L.G. Tiu, L. Li, and B. Brown. 2008 Marker-assisted cohort selection of yellow perch for improvement of growth rate. Proceedings of the 2008 World Aquaculture Society Conference. Orlando.
• Yao, H, H.P. Wang, G.K. Wallat, L.G. Tiu, P. OBryant and J.D. Rapp. 2008. Evaluation of growth performance of different families in individual and communal tanks using microsatellite tool. Proceedings of the 2008 World Aquaculture Society Conference. Orlando.
• Wang, H.P, G. K. Wallat, Li Li, L. G. Tiu, J. D. Rapp, P.OBryant, and B.B. Brown. 2007. Integrated genetic improvement of yellow perch broodstock through marker assisted selection. Proceedings of the 2007 World Aquaculture Society Conference, page 965.
• Li Li, Chandler B. Givens, Han-Ping Wang, Geoff K. Wallat, Laura G. Tiu and Bonnie L. Brown. 2007. Genetic variation among yellow perch perca flavescens broodstock groups: effects of spawning and management practices to date. Proceedings of the 2007 World Aquaculture Society Conference, page 524.
• Li Li, Hong Yao, Han-Ping Wang, Geoff K. Wallat, Laura G. Tiu, Jacob Paul OBryant, Dean Rapp and Bonnie L. Brown. 2007. Microsatellite-based family identification, offspring fitness comparison, and environmental effects in communally reared yellow perch Perca flavescens. . Proceedings of the 2007 World Aquaculture Society Conference, page 525.
• Reddish, J.M., K.B. Green-Church, A.D. Nichols, N. St-Pierre, and M. Wick. 2007. Proteomic analysis of whole muscle fingerprints from yellow perch, Perca flavescens, and identification of proteins associated with body weight and length. Abstract # 22732, Proceedings of the 2007 Joint ADSA-PSA-AMPA-ASAS Meeting, July 8-12, 2007, San Antonio, Texas.
• Williams, H., J. M. Reddish, M. Wick. 2006. Purification of Myosin Isoforms from Epaxial Muscle of Yellow Perch (Perca flavecens). Poster presentation Richard J. and Martha D. Denman Undergraduate Research Forum, 2007.
• Williams, H., J. M. Reddish, M. Wick. 2007. Purification of Myosin Isoforms from Epaxial Muscle of Yellow Perch (Perca flavecens). Poster CFAES Undergraduate Research Forum.
• Brown, B., H. P Wang, L. Li, G. Chandler, and G.K. Wallat. 2007. Yellow perch strain evaluation I. Genetic variance of six broodstock populations. Aquaculture 271:142-151.
• Li, L. H. P. Wang, C. Givens, and B. Brown. 2007. Isolation and characterization of microsatellites in the yellow perch (Perca flavescens). Molecular Ecology Notes 7: 600-603.

Progress 09/15/08 to 09/14/09

Outputs
OUTPUTS: Activities: From selected broodfish of 2005 year-class, the 2nd generation of improved fish was created in 2009. On-station testing is underway to test the performance of the second generation of improved fish vs. control. Genotyping for selected yellow perch broodfish was completed and genetic pedigree was constructed using microsatellite parentage analysis for the breeding. For bluegill, about 15,000 all-male and 5,000 super male populations were reared to maturation for developing all-male broodstock in 2009. A first linkage map of bluegill was constructed for male and female for detection of important commercial traits in the future. We completed a project "Establishment of mostly-male groups of bluegill by size-selection and evaluation of their growth performance". For the Bowling Green Aquaculture Program, preliminary trials with on-farm cooperators has shown that another baitfish species, the golden shiner, can be successfully raised to market size in one growing season. Production has ranged from 800 to over 1200 pounds per acre, with wholesale prices of $3.00 / lb. Preliminary pond trials with spotfin shiners at a cooperator's ponds demonstrated they can be successfully grown to market size (2 in) in one growing season. Four shrimp farmers participated in hands-on training for water quality monitoring equipment, and recommendations on feeding and husbandry. We continued teaching and mentoring of freshwater shrimp farmers in Ohio during the 2008 and 2009 growing seasons. An assessment of the Ohio State University Aquaculture Program (OSUAP) is under development. A survey, to be launched in December 2009, will be utilized to describe Ohio Fish Farmers and explore their participation with and perceived impact of OSUAP products. Information will also be collected to determine stakeholder needs for the future. Events: A freshwater prawn symposium was part of the 2009 Ohio Aquaculture Association Annual Meeting. Services: Site visits, personal and phone consulting regarding all aspects of freshwater fish and shrimp culture in Ohio are available during business hours. At the Bowling Green Aquaculture Center, additional technical support was provided throughout the growing season through site visits and weekly phone calls. Products: Two groups of the 2nd generation of improved fish have been created in 2009. A linkage map of bluegill was constructed. A webpage (http://www.ag.ohio-state.edu/~prec/aqua/shrimp.htm) with links to freshwater shrimp information has been created and is available to clients 24/7. PARTICIPANTS: Ottobre, J. S. Director of project. Overall coordination. Wang, H. P., Director of GIFT (Genetic Improvement of Farmed-fish Traits) Program. Wallat, G,. Director of Bowling Green Aquaculture Program. Tiu, L. G., Director of Aquaculture Technology Program. Wick, M., Director of Fish Muscle Growth and Nutrition Program. TARGET AUDIENCES: Target audiences are aquacultural producers in Ohio. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The selected generations of superior broodfish consolidated the foundation for breeding programs for yellow perch and bluegill. The improved growth rate of 25-54% of selected yellow perch lines vs. controls shows a great premise of the breeding program and potential impact on the yellow perch industry. A first linkage map of bluegill was published in Aquaculture International (2009) and laid a foundation for detection of important commercial traits in the future for the bluegill. A practical strategy for establishing mostly-male bluegill groups that can gain 61.0% more weight than the mixed-sex control was published in North American Journal of Aquaculture (2009). Bowling Green research site provides a physical demonstration facility of RAS technology for clientele in the northwest Ohio region, as well as the personnel with experience in RAS to provide outreach training and consultation. Preliminary investigations showed that market-size spotfin shiners and golden shiners can be raised in one growing season in Ohio's temperate climate. This success has spurred interest from other growers, looking to expand or develop new aquaculture markets in Ohio. A new commercial-scale golden shiner incubation system was developed, improving hatch out and swim up survival to 75%. Clients experience a change in knowledge when they access the services or information available at OARDIP. The freshwater shrimp industry has expanded to approximately 25 growers in 2009, up from 2 growers in 2000. The entire project has had import impacts on Ohio aquaculture through research and technology transfer. Aquaculture sales in Ohio have tripled from $1.8 million to $6.6 million in recent years. Nationally, Ohio ranks first in sales of yellow perch for food and is the number one bluegill producing state. Ohio also ranks fourth in sales of baitfish and largemouth bass sold for sport and fifth in number of baitfish farms.

Publications

• Wang, H.P. G.K. Wallat, R. Hayward, L.G. Tiu, P. O'Bryant and D. Rapp. 2009. Establishment of mostly-male groups of bluegill by size-selection and evaluation of their growth performance. North American Journal of Aquaculture, 71:216 - 223.
• Wang , W.J., H.P. Wang, L. Li, G.K. Wallat, L.G. Tiu, H.Yao, and Q.Y. Wang. 2009. A first genetic linkage map of bluegill sunfish (Lepomis macrochirus) using AFLP markers. Aquaculture International, DOI 10.1007/s10499-009-9303-1 (published online: http://www.springerlink.com/content/164383pvn1r224r7/fulltext.pdf).

Progress 09/15/07 to 09/14/08

Outputs
OUTPUTS: Activities: Genotyping for selected yellow perch broodfish from the 2006 year-class was completed using microsatellite analysis. Combined genetic relatedness analysis of selected 2005 and 2006 class fish was completed and a large color-coded chart of relatedness was constructed in spring 2008. By crossing selected broodfish of 2006 year-class based on the chart, the second generation of improved fish was created in 2008. On-station testing is underway to test the performance of the first and second generation of improved fish vs. control. 2007 year-class fish have been reared to maturation for next round of broodfish selection. For bluegill, the success of sex-reversing regular fish to all females was published in Aquaculture. About 15,000 all-male and 5,000 super male populations, which would grow 40-50 percent faster than a mixed-gender population, have been generated for developing all-male broodstock in 2008. This is the first time that an all male bluegill population has been achieved. In studies of yellow perch muscle, we continue to make significant advances to our novel high throughput functional proteomic methodology. This method allows for the identification of multiple classes of proteins that potentially participate in skeletal muscle growth in aquatic species. For Bowling Green Aquaculture Program, preliminary trials with on-farm cooperators has shown that another baitfish species, the golden shiner, can be successful raised to market size in one growing season. Production has ranged from 800 to over 1200 pounds per acre, with wholesale prices of 3 dollars per pound. Four shrimp farmers participated in hands-on training for water quality monitoring equipment, and recommendations on feeding and husbandry. We continued teaching and mentoring of freshwater shrimp farmers in Ohio during the 2008 growing season. Events: An in-depth training session on commercial recirculating aquaculture systems (RAS) was conducted at the Bowling Green Aquaculture Center for 20 participants, and the on-site researcher has participated one-on-one with over 30 clients interested in RAS and has helped several clients in their design and implementation of these systems. In conjunction with Ohio Aquaculture Association, a freshwater shrimp culture workshop was conducted in 2008. One shrimp producer held training workshops on his farm during the spring of 2008 using materials developed by OCARD. Services: Site visits, personal and phone consulting regarding all aspects of freshwater fish and shrimp culture in Ohio are available during business hours. In Bowling Green Aquaculture Center, additional technical support was provided throughout the growing season through site visits and weekly phone calls. Four additional farmers were identified as a "control" group, who would only receive standard outreach support. Products: Five groups of the second generation of improved fish have been created in 2008. A linkage map of bluegill was constructed and a manuscript on this is in review. A webpage (www.ag.ohio-state.edu/~prec/aqua/shrimp.htm) with links to freshwater shrimp information has been created and is available to clients 24/7. PARTICIPANTS: Ottobre, J. S. Director of project. Overall coordination. Wang, H. P. Director of genetic improvement initiatives in yellow perch and bluegill. Wallat, G. Director of Bowling Green activities. Tiu, L. Director of outreach efforts. Wick, M. Director of muscle biology and muscle proteomics. TARGET AUDIENCES: Target audiences are aquacultural producers in Ohio. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The selected first generation of superior broodfish consolidated the foundation for a marker-assisted breeding program for yellow perch. The improved growth rate of 25-54 percent of improved lines vs. controls shows a great premise of the breeding program and potential impact on the yellow perch industry. Bowling Green research site provides a physical demonstration facility of RAS technology for clientele in the northwest Ohio region, as well as the personnel with experience in RAS to provide outreach training and consultation. The preliminary investigation showed that market-size golden shiners can be raised in one growing season in Ohio's temperate climate. This success has spurred interest from other growers, looking to expand or develop new aquaculture markets in Ohio. Clients experience a change in knowledge when they access the services or information available at OCARD. The freshwater shrimp industry has expanded to approximately 25 growers in 2008 up from 2 growers in 2000.

Publications

• Williams, H., J. M. Reddish, M. Wick. 2007. Purification of Myosin Isoforms from Epaxial Muscle of Yellow Perch (Perca flavecens). Poster presentation Richard J. and Martha D. Denman Undergraduate Research Forum, 2007.
• Williams, H., J. M. Reddish, M. Wick. 2007. Purification of Myosin Isoforms from Epaxial Muscle of Yellow Perch (Perca flavecens). Poster CFAES Undergraduate Research Forum.
• Patent submission to the Office of Technology Transfer. 2008. Multiple linear regression proteomics, and have assigned Tech ID # 08085F
• Wang, H.P. Z. Gao, B. Beres, J. Ottobre, G.K. Wallat, L.G. Tiu, D. Rapp, P. O Bryant and H. Yao. 2008. Effects of estradiol-17 on survival, growth performance, sex reversal and gonadal structure of bluegill sunfish Lepomis macrochirus. Aquaculture 285: 216-223
• Wang, H.P., G.K. Wallat, P. O Bryant, J. D. Rapp, H. Yao, L.G. Tiu, L. Li, and B. Brown. 2008 Marker-assisted cohort selection of yellow perch for improvement of growth rate. Proceedings of the 2008 World Aquaculture Society Conference. Orlando.
• Yao, H, H.P. Wang, G.K. Wallat, L.G. Tiu, P. O Bryant and J.D. Rapp. 2008. Evaluation of growth performance of different families in individual and communal tanks using microsatellite tool. Proceedings of the 2008 World Aquaculture Society Conference. Orlando.

Progress 09/15/06 to 09/14/07

Outputs
With respect to the objective relating to the genetic improvement program of yellow perch, approximately 800 best fish were selected from 24,000 fish of 2005 year-class families as broodfish of the next generation. Genotyping for selected broodfish from the 2005 year-class was completed using microsatellite analysis. Combined genetic relatedness analysis of selected 2004 and 2005 class fish was completed and a large color-coded chart of relatedness was constructed in spring 2007. By crossing selected broodfish of 2004 and 2005 year-class based on the chart, five improved lines with approximately 105,000 fingerlings were achieved. Seven experiments in small tanks, large tanks and research ponds, and an on-farm testing are being carried out to test the performance of these progeny produced in spring of 2006 and 2007. The data from the large tank experiment that has been run for 15 months shows that the two improved lines of 2006 grew 54% (line 1) and 28% (line 2) faster than controls. The preliminary data for 2007 progeny shows genetic lines grew 25% - 42% faster than controls. Parentage analysis techniques using six molecular markers in yellow perch have been developed and family identification for 2004 and 2005 year-class perch reared in communal ponds was completed. Two mapping families have been established and reared to maturation in tanks for next year's quality trait loci mapping. Our electrophoretic proteomic methodology concentrated on developing a novel high throughput functional proteomic analysis to identify muscle proteins associated with body weight and length in yellow perch. The manuscript, "Proteomic analysis of whole muscle from yellow perch, Perca flavescens: Identification of proteins associated with body mass and length" has been submitted to a peer-reviewed journal and is currently in revision. A marketing survey for Ohio retail bait stores is being developed for initiation in the winter of 2007. Based on preliminary investigation, one Ohio farmer cooperated with the OSU program to rear golden shiners in 2007. He produced over 800 pounds of shiners, in one season, which were all marketed to a local grower. An in-depth training session on commercial recirculating aquaculture systems (RAS) was conducted at the Bowling Green site for 20 participants. The on-site researcher at Bowling Green has participated one-on-one with over 30 clients interested in RAS and has helped several clients in their design and implementation of these systems. Four shrimp farmers participated in hands-on training for water quality monitoring equipment, and recommendations on feeding and husbandry. Additional technical support was provided throughout the growing season through site visits and weekly phone calls. Four additional farmers were identified as a "control" group, which would only receive standard outreach support. All farmers will be surveyed in the winter 2007 on their growing season and satisfaction with the support and training made available to them.

Impacts
The selected first generation of superior broodfish consolidated the foundation for a marker-assisted breeding program for yellow perch. The superior growth rate of 25-54% of improved lines vs. controls shows a great premise of the breeding program and potential impact on the yellow perch industry. The preliminary investigation showed that market-size golden shiners can be raised in one growing season in Ohio's temperate climate. This success has spurred interest from other growers, looking to expand or develop new aquaculture markets in Ohio. The Bowling Green research site provides a physical demonstration facility of RAS technology for clientele in the northwest Ohio region, as well as the personnel with experience in RAS to provide outreach training and consultation. Testing of several types of commercial RAS at the Bowling Green site has provided clients with information on which systems will work best in their operation. Initial comments from the shrimp farmers receiving additional training and support are that the service was very helpful to their success. Survey results will assist in measuring the impact of advanced outreach support and to design targeted outreach efforts that produce results. The workshops and outreach training on commercial recirculating aquaculture will lead to better management and production efficiencies for Ohio aquaculture operations.

Publications

• Brown, B., H. P Wang, L. Li, G. Chandler, and G.K. Wallat. 2007. Yellow perch strain evaluation I. Genetic variance of six broodstock populations. Aquaculture 271:142-151.
• Li, L. H. P. Wang, C. Givens, and B. Brown. 2007. Isolation and characterization of microsatellites in the yellow perch (Perca flavescens). Molecular Ecology Notes 7: 600-603.
• Wang, H.P, G. K. Wallat, Li Li, L. G. Tiu, J. D. Rapp, P.O'Bryant, and B.B. Brown. 2007. Integrated genetic improvement of yellow perch broodstock through marker assisted selection. Proceedings of the 2007 World Aquaculture Society Conference, page 965.
• Li Li, Chandler B. Givens, Han-Ping Wang, Geoff K. Wallat, Laura G. Tiu and Bonnie L. Brown. 2007. Genetic variation among yellow perch perca flavescens broodstock groups: effects of spawning and management practices to date. Proceedings of the 2007 World Aquaculture Society Conference, page 524.
• Li Li, Hong Yao, Han-Ping Wang, Geoff K. Wallat, Laura G. Tiu, Jacob Paul O'Bryant, Dean Rapp and Bonnie L. Brown. 2007. Microsatellite-based family identification, offspring fitness comparison, and environmental effects in communally reared yellow perch Perca flavescens. Proceedings of the 2007 World Aquaculture Society Conference, page 525.
• Reddish, J.M., K.B. Green-Church, A.D. Nichols, N. St-Pierre, and M. Wick. 2007. Proteomic analysis of whole muscle fingerprints from yellow perch, Perca flavescens, and identification of proteins associated with body weight and length. Abstract # 22732, Proceedings of the 2007 Joint ADSA-PSA-AMPA-ASAS Meeting, July 8-12, 2007, San Antonio, Texas.

Progress 09/15/05 to 09/15/06

Outputs
For genetic selection of yellow perch, approximately 700 best fish were selected from 18,000 fish of 04 year-class families, and 800 best fish selected from 24,000 fish of 05 year-class families as broodfish of the next generation. Genetic relatedness analysis of selected 04 class fish was completed and a new color-coded chart of relatedness was constructed. By crossing selected broodfish of 04 year-class using the chart, two improved lines with approximately 95,700 fingerlings were achieved. Four experiments in small tanks, large tanks, research ponds, and commercial ponds are been carried out to test the performance of these progeny. The preliminary data from the small tank experiment with four replicates shows genetic gains of the two lines; fish grew 39.8% (line 1) and 18.3% (line 2) faster than controls. Family identification techniques using molecular makers in yellow perch has been developed and family identification for 2004 year-class perch reared in communal ponds has been completed at the Aquaculture Genetics and Breeding Laboratory at Piketon. So far 90% of the progeny was unambiguously assigned to the parents. Thirteen pairs of matings were made between the best and worst performing fish for mapping families in the Spring and two of these pairs are being reared in tanks for future QTL mapping. A portion of selected broodfish from the 05 class has been genotyped using microsatellite analysis and the database will be used for selective breeding next year. For YY-bluegill selection, larvae nursery technique with artificial feed has been established at Piketon, and two new batches of juveniles have been produced using the technique. A new sex reversal experiment to produce another batch of XY-females is underway. The first batch of sex-reversed bluegill is being induced to maturity and will produce partial YY-bluegill next spring. We have already set up the AFLP (Amplified Fragment Length Polymorphism) technique in bluegill to study sex-linked markers for future sexual identification. There are 17 female and 4 male sex-specific markers detected by a DNA pool strategy. The selected candidate sex-specific markers with DNA pool have been subsequently confirmed with individual fish. We have developed a 2D electrophoretic proteomic methodology, thus increasing the number of proteins identified associated with muscle body weight and length in yellow perch by a factor of 10. New information concerning the nutritional requirements of cultured myoblasts has been generated by our collaborators at WSU. For Bowling Green objectives, the outreach training on commercial recirculating aquaculture systems were held on December 9, 2006, with 30 students registered. The Freshwater Shrimp culture training program will begin February 2007, and continue through September 2007. Ten participants have been identified to receive the in-depth outreach training for freshwater shrimp culture during this time period.

Impacts
The results from strain evaluation and year one selective breeding will allow us to establish the foundation for a marker-assisted breeding program, which will expect to improve growth rate by 15-20% per generation. The new information from in vitro and in vivo muscle studies will continue to be useful in identifying gene products unique to enhanced muscle growth and development. This information will allow for producers to develop useful breeding strategies for the production of yellow perch. The Bowling Green program will have substantial effects on aquaculture operations on northwest and central lake counties in Ohio. The outreach training on commercial recirculating aquaculture systems and the Freshwater Shrimp culture training program will lead to better management and production efficiencies for Ohio aquaculture operations, and these impacts will be documented through survey collection and written feedback.

Publications

• No publications reported this period