Progress 09/01/09 to 08/31/13

Outputs
Target Audience: Food animal producers, other animal owners, veterinary practitioners, Extension Veterinarians, State and Federal Animal Health Officials, the animal health industry. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? OUTPUTS: During the 2009-2010 hunting season the SDSU ADRDL joined with the South Dakota Game Fish and Parks Department and Wildlife Services to test >650 wild birds for Avian Influenza Virus infection, using the PCR test (Avian Influenza matrix screen) followed, in the case of a positive matrix test result, by H5 and H7 PCR tests. The SDSU ADRDL also tests wild bird samples from other states as a member of the NAHLN. The lab continues to work with the state meat inspection programs of ND and SD to test all farm raised cervids such as deer and elk for Chronic Wasting Disease (CWD) via the immunohistochemistry (IHC) test. ADRDL also performs Scrapie IHC testing for the various regulatory programs designed to aid in the eradication of Scrapie. In addition to the regulatory programs, ADRDL has joined with South Dakota Game Fish and Parks Department during the 2009-2010 deer and elk hunting seasons, to perform surveillance testing for CWD utilizing the BioRad ELISA with confirmatory CWD IHC testing. To date, the lab has completed the following surveillance tests for this fiscal year (July 1 2009 thru June 15, 2010): Exotic Newcastle Disease-98 PCR tests; Avian Influenza Matrix-1396 PCR tests; Avian Influenza-792 Serology tests; Avian Influenza H5/H7-508 PCR tests; Swine Influenza Virus-1516 Serology tests; Swine Influenza Virus-89 PCR tests; Swine Influenza Virus N1-2 PCR tests; Foot & Mouth Disease-98 PCR tests; Classical Swine Fever-330 PCR tests; Chronic Wasting Disease-840 ELISA tests; Scrapie-955 IHC tests. With regard to training, IT / NAHLN development training has included attendance by the ADRDL IT specialist, the ADRDL quality manager, and faculty at the following meetings: ABSL3Ag and ABSL3 - Special Species (ABSL3+). Atlanta, Georgia. January 24, 2010. (Tanya Graham) AAVLD Laboratory Safety Committee Symposium. San Diego, California. October 11, 2009. (Tanya Graham; Rajesh Parmar) American Association of Veterinary Laboratory Diagnosticians 52th Annual Conference. San Diego, California. Oct. 10-12, 2009. (Rajesh Parmar; David Zeman; Jon Greseth; Regg Neiger; Tanya Graham) AAVLD Accreditation & Quality Assurance Symposium. San Diego, California. October 8, 2009. (Rajesh Parmar, Tanya Graham) To date we have successfully sent test messages to the NAHLN repository computer and have received test messages from NAHLN. We also recently completed upgrades of the VADDS table structure to handle necessary NAHLN data fields. Refinements such as this remove barriers relative to VADDS interfacing with the NAHLN, both at our laboratory and at other diagnostic laboratories utilizing the VADDS laboratory information management system. PARTICIPANTS: Not relevant to this project. OUTPUTS: OUTPUTS: During the 2010-2011 hunting season the SDSU ADRDL joined with the South Dakota Game Fish and Parks Department and Wildlife Services to test more than 1000 wild birds for Avian Influenza Virus infection, using the PCR test (Avian Influenza matrix screen) followed, in the case of a positive matrix test result, by H5 and H7 PCR tests. The SDSU ADRDL also tests wild bird samples from other states as a member of the National Animal Health Laboratory Network (NAHLN). The lab continues to work with the state meat inspection programs of ND and SD to test all farm raised cervids such as deer and elk for Chronic Wasting Disease (CWD) via the immunohistochemistry (IHC) test. ADRDL also performs Scrapie IHC testing for the various regulatory programs designed to aid in the eradication of Scrapie. In addition to the regulatory programs, ADRDL has joined with South Dakota Game Fish and Parks Department during the 2010-2011 deer and elk hunting seasons, to perform surveillance testing for CWD utilizing the BioRad ELISA with confirmatory CWD IHC testing. During the period from 07/01/10 thru 06/30/11, the following surveillance tests were performed at ADRDL: Exotic Newcastle Disease-96 PCR tests; Avian Influenza Matrix-1041 PCR tests; Avian Influenza-687 Serology tests; Avian Influenza H5/H7- 207 PCR tests; Swine Influenza Virus-2388 Serology tests; Swine Influenza Virus & Novel H1 tests-1149 PCR tests; Foot & Mouth Disease-330 PCR tests; Classical Swine Fever-245 PCR tests; Chronic Wasting Disease-1780 ELISA tests; Scrapie and CWD-804 IHC tests. With regard to training, IT / NAHLN training, members of ADRDL have attended the following meetings: American Association of Veterinary Laboratory Diagnosticians 53rd Annual Conference. Minneapolis, Minnesota. Nov. 11-17, 2010. To date we have successfully sent test messages to the NAHLN repository computer and have received test messages from NAHLN. We also recently completed upgrades of the VADDS table structure to handle necessary NAHLN data fields. Refinements such as this remove barriers relative to VADDS interfacing with the NAHLN, both at our laboratory and at other diagnostic laboratories utilizing the VADDS laboratory information management system. PARTICIPANTS: Dr. David H. Zeman (PI); Dr. Tanya D. Graham, Mr. Rajesh Parmar, and Mr. Jon Greseth (key personnel); and the diagnosticians and technicians of the South Dakota Animal Disease Research & Diagnostic Laboratory. How have the results been disseminated to communities of interest? Results are dissminated to stakeholders (veterinarians, regulatory, and industry groups) through the ADRDL Annual Report. Timely articles of interest are published in the departmental newsletter and through the departmental website. What do you plan to do during the next reporting period to accomplish the goals? Continue to maintain staff and facility infrastructure in order to provide accurate timely answers to diagnostic questions (in the form of diagnostic submissions) by veterinarians.

Impacts
What was accomplished under these goals? The goals of this project are to enhance the capability and capacity of State and University animal disease diagnostic laboratories to perform/provide 1) surveillance for routine and exotic/foreign animal diseases, 2) early detection of foreign animal diseases, 3) a rapid response to disease outbreaks, and 4) the appropriate and timely recovery from an animal agriculture catastrophe. The presence of a network of diagnostic laboratories means that facilities, personnel, and expertise are available nationwide, no matter where a disease outbreak occurs. The mission statement of the National Animal Health Laboratory Network (NAHLN) includes the following: provide timely, accurate and consistent services nationwide with a focus on exotic, zoonotic, and emerging diseases of livestock and non-livestock species; provide laboratory data to meet epidemiological and disease reporting needs, and to maintain the laboratory network capacity and capability in order to respond to foreign animal disease outbreaks or other adverse animal health events. This includes both routine testing for endemic diseases (e.g. Swine Influenza Virus) as well as active surveillance for foreign animal diseases such as Foot and Mouth Disease, Classical Swine Fever, Exotic Newcastle Disease, and Influenza viruses in swine and poultry. Surveillance for diseases of public health interest such as the prion-associated diseases (Bovine Spongiform Encephalopathy in cattle, Chronic Wasting Disease of deer and elk, and Scrapie disease in sheep) and novel (aka: pandemic) H1N1 influenza virus in swine are also an important facet of the animal disease surveillance provided by the NAHLN. Disease examinations were conducted on 19,975 diagnostic case submissions during FY11. Microbiological, chemical, serological, and pathological tests totaling 442,630 were performed on the submitted specimens. Bovine and porcine submissions made up the bulk of the caseload. Evaluation of diagnostic case materials submitted to the Animal Disease Research and Diagnostic Laboratory (ADRDL) provides valuable surveillance of emerging and re-emerging diseases. Common disease syndromes encountered included respiratory disease, neonatal enteritis, and spontaneous abortions in food animals. In addition, 502 rabies examinations were performed on a variety of animal species. There were 35 positive rabies cases identified - 18 were in skunks, 2 in bats, 7 in cattle, 2 in dogs, 4 in feline, and 2 in raccoons. In 29 of the positive rabies cases there was no known human exposure; but in 6 of the positive cases human exposure was documented. During the year, 57,403 molecular diagnostic assays were performed. This resulted in identifying 94 cases of Johne's Disease in cattle, 241 cases of BVD virus in cattle, 501 cases of Circovirus type 2 infection in swine, 3,500 cases of PRRS virus infection in swine and 443 cases of Swine Influenza. The serology section of the ADRDL conducted 323,435 serological assays that are useful to detect exposed or carrier animals. The laboratory's expertise is nationally recognized, especially relative to PRRS virus detection; 80,196 PRRS virus ELISA assays were performed. Veterinary diagnostic investigations of active disease outbreaks require skilled multidisciplinary and comprehensive examinations of sick and/or dead animals to produce accurate diagnoses. These investigations are coordinated by veterinary diagnostic pathologists. The ADRDL pathologists, and necropsy and histopathology teams processed 1,527 necropsy cases and evaluated 15,797 tissue sections. PARTICIPANTS: The work was performed by faculty and staff of the South Dakota State University Animal Disease Research and Diagnostic Laboratory. Partners include the USDA National Animal Health Laboratory Network. TARGET AUDIENCES: Food animal producers, other animal owners, veterinary practitioners, Extension Veterinarians, State and Federal Animal Health Officials, the animal health industry. PROJECT MODIFICATIONS: Not relevant to this project. Monitoring disease activity in domestic and wild animals is important to control costs of food production and to minimize potential zoonotic disease occurrence in people. Disease examinations were conducted on 19,975 diagnostic case submissions during FY12. Microbiological, chemical, serological, and pathological tests totaling 591,014 were performed on the submitted specimens. Bovine and porcine submissions made up the bulk of the caseload. Evaluation of diagnostic case materials submitted to the Animal Disease Research and Diagnostic Laboratory (ADRDL) provides valuable surveillance of emerging and re-emerging diseases. Common disease syndromes encountered included respiratory disease, neonatal enteritis, and spontaneous abortions in food animals. In addition, 554 rabies examinations were performed on a variety of animal species. There were 33 positive rabies cases identified - 13 were in skunks, 4 in bats, 11 in cattle, 1 in dogs, 0 in feline, and 0 in raccoons. In 28 of the positive rabies cases there was no known human exposure; but in 5 of the positive cases human exposure was documented. During the year, 62,769 molecular diagnostic assays were performed. This resulted in identifying 147 cases of Johne's Disease in cattle, 312 cases of BVD virus in cattle, 128 cases of Circovirus type 2 infection in swine, 4,136 cases of PRRS virus infection in swine and 1,080 cases of Swine Influenza. The serology section of the ADRDL conducted 482,024 serological assays that are useful to detect exposed or carrier animals. The laboratory's expertise is nationally recognized, especially relative to PRRS virus detection; 52,486 PRRS virus ELISA assays were performed. Veterinary diagnostic investigations of active disease outbreaks require skilled multidisciplinary and comprehensive examinations of sick and/or dead animals to produce accurate diagnoses. These investigations are coordinated by veterinary diagnostic pathologists. The ADRDL pathologists, and necropsy and histopathology teams processed 2,959 necropsy cases and evaluated 12,358 tissue sections.

Publications

• Type: Other Status: Published Year Published: 2011 Citation: David H. Zeman and Staff. South Dakota Animal Disease Research and Diagnostic Laboratory Annual Report. 2011. 116 pages, SDSU Print Lab.
• Type: Journal Articles Status: Published Year Published: 2010 Citation: Lawson, S., J. Lunney, F. Zuckermann, F. Osaorio, E. Nelson, C. Welbon, T. Clement, Y. Fang, S. Wong, K. Kulas, and J. Christopher-Hennings. 2010. Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: Assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination. Vaccine 28(32):5356-5364.
• Type: Other Status: Published Year Published: 2012 Citation: David H. Zeman and staff. South Dakota Animal Disease Research and Diagnostic Laboratory Annual Report, 2012. 122 pg., SDSU Print Lab

Progress 08/31/12 to 08/30/13

Outputs
Target Audience: Food animal producers, other animal owners, veterinary practitioners, Extension Veterinarians, State and Federal Animal Health Officials, the animal health industry. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Results are dissminated to stakeholders (veterinarians, regulatory, and industry groups) through the ADRDL Annual Report. Timely articles of interest are published in the departmental newsletter and through the departmental website. What do you plan to do during the next reporting period to accomplish the goals? Continue to maintain staff and facility infrastructure in order to provide accurate timely answers to diagnostic questions (in the form of diagnostic submissions) by veterinarians.

Impacts
What was accomplished under these goals? Monitoring disease activity in domestic and wild animals is important to control costs of food production and to minimize potential zoonotic disease occurrence in people. Disease examinations were conducted on 19,975 diagnostic case submissions during FY12. Microbiological, chemical, serological, and pathological tests totaling 591,014 were performed on the submitted specimens. Bovine and porcine submissions made up the bulk of the caseload. Evaluation of diagnostic case materials submitted to the Animal Disease Research and Diagnostic Laboratory (ADRDL) provides valuable surveillance of emerging and re-emerging diseases. Common disease syndromes encountered included respiratory disease, neonatal enteritis, and spontaneous abortions in food animals. In addition, 554 rabies examinations were performed on a variety of animal species. There were 33 positive rabies cases identified - 13 were in skunks, 4 in bats, 11 in cattle, 1 in dogs, 0 in feline, and 0 in raccoons. In 28 of the positive rabies cases there was no known human exposure; but in 5 of the positive cases human exposure was documented. During the year, 62,769 molecular diagnostic assays were performed. This resulted in identifying 147 cases of Johne's Disease in cattle, 312 cases of BVD virus in cattle, 128 cases of Circovirus type 2 infection in swine, 4,136 cases of PRRS virus infection in swine and 1,080 cases of Swine Influenza. The serology section of the ADRDL conducted 482,024 serological assays that are useful to detect exposed or carrier animals. The laboratory's expertise is nationally recognized, especially relative to PRRS virus detection; 52,486 PRRS virus ELISA assays were performed. Veterinary diagnostic investigations of active disease outbreaks require skilled multidisciplinary and comprehensive examinations of sick and/or dead animals to produce accurate diagnoses. These investigations are coordinated by veterinary diagnostic pathologists. The ADRDL pathologists, and necropsy and histopathology teams processed 2,959 necropsy cases and evaluated 12,358 tissue sections.

Publications

• Type: Other Status: Published Year Published: 2012 Citation: David H. Zeman and staff. South Dakota Animal Disease Research and Diagnostic Laboratory Annual Report, 2012. 122 pg., SDSU Print Lab

Progress 09/01/11 to 08/30/12

Outputs
OUTPUTS: Disease examinations were conducted on 19,975 diagnostic case submissions during FY11. Microbiological, chemical, serological, and pathological tests totaling 442,630 were performed on the submitted specimens. Bovine and porcine submissions made up the bulk of the caseload. Evaluation of diagnostic case materials submitted to the Animal Disease Research and Diagnostic Laboratory (ADRDL) provides valuable surveillance of emerging and re-emerging diseases. Common disease syndromes encountered included respiratory disease, neonatal enteritis, and spontaneous abortions in food animals. In addition, 502 rabies examinations were performed on a variety of animal species. There were 35 positive rabies cases identified - 18 were in skunks, 2 in bats, 7 in cattle, 2 in dogs, 4 in feline, and 2 in raccoons. In 29 of the positive rabies cases there was no known human exposure; but in 6 of the positive cases human exposure was documented. During the year, 57,403 molecular diagnostic assays were performed. This resulted in identifying 94 cases of Johne's Disease in cattle, 241 cases of BVD virus in cattle, 501 cases of Circovirus type 2 infection in swine, 3,500 cases of PRRS virus infection in swine and 443 cases of Swine Influenza. The serology section of the ADRDL conducted 323,435 serological assays that are useful to detect exposed or carrier animals. The laboratory's expertise is nationally recognized, especially relative to PRRS virus detection; 80,196 PRRS virus ELISA assays were performed. Veterinary diagnostic investigations of active disease outbreaks require skilled multidisciplinary and comprehensive examinations of sick and/or dead animals to produce accurate diagnoses. These investigations are coordinated by veterinary diagnostic pathologists. The ADRDL pathologists, and necropsy and histopathology teams processed 1,527 necropsy cases and evaluated 15,797 tissue sections. PARTICIPANTS: The work was performed by faculty and staff of the South Dakota State University Animal Disease Research and Diagnostic Laboratory. Partners include the USDA National Animal Health Laboratory Network. TARGET AUDIENCES: Food animal producers, other animal owners, veterinary practitioners, Extension Veterinarians, State and Federal Animal Health Officials, the animal health industry. PROJECT MODIFICATIONS: Not relevant to this project.

Impacts
Monitoring disease activity in domestic and wild animals is important to control costs of food production and to minimize potential zoonotic disease occurrence in people.

Publications

• David H. Zeman and Staff. South Dakota Animal Disease Research and Diagnostic Laboratory Annual Report. 2011. 116 pages, SDSU Print Lab.
• Lawson, S., J. Lunney, F. Zuckermann, F. Osaorio, E. Nelson, C. Welbon, T. Clement, Y. Fang, S. Wong, K. Kulas, and J. Christopher-Hennings. 2010. Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: Assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination. Vaccine 28(32):5356-5364.

Progress 09/01/10 to 08/31/11

Outputs
OUTPUTS: OUTPUTS: During the 2010-2011 hunting season the SDSU ADRDL joined with the South Dakota Game Fish and Parks Department and Wildlife Services to test more than 1000 wild birds for Avian Influenza Virus infection, using the PCR test (Avian Influenza matrix screen) followed, in the case of a positive matrix test result, by H5 and H7 PCR tests. The SDSU ADRDL also tests wild bird samples from other states as a member of the National Animal Health Laboratory Network (NAHLN). The lab continues to work with the state meat inspection programs of ND and SD to test all farm raised cervids such as deer and elk for Chronic Wasting Disease (CWD) via the immunohistochemistry (IHC) test. ADRDL also performs Scrapie IHC testing for the various regulatory programs designed to aid in the eradication of Scrapie. In addition to the regulatory programs, ADRDL has joined with South Dakota Game Fish and Parks Department during the 2010-2011 deer and elk hunting seasons, to perform surveillance testing for CWD utilizing the BioRad ELISA with confirmatory CWD IHC testing. During the period from 07/01/10 thru 06/30/11, the following surveillance tests were performed at ADRDL: Exotic Newcastle Disease-96 PCR tests; Avian Influenza Matrix-1041 PCR tests; Avian Influenza-687 Serology tests; Avian Influenza H5/H7-207 PCR tests; Swine Influenza Virus-2388 Serology tests; Swine Influenza Virus & Novel H1 tests-1149 PCR tests; Foot & Mouth Disease-330 PCR tests; Classical Swine Fever-245 PCR tests; Chronic Wasting Disease-1780 ELISA tests; Scrapie and CWD-804 IHC tests. With regard to training, IT / NAHLN training, members of ADRDL have attended the following meetings: American Association of Veterinary Laboratory Diagnosticians 53rd Annual Conference. Minneapolis, Minnesota. Nov. 11-17, 2010. To date we have successfully sent test messages to the NAHLN repository computer and have received test messages from NAHLN. We also recently completed upgrades of the VADDS table structure to handle necessary NAHLN data fields. Refinements such as this remove barriers relative to VADDS interfacing with the NAHLN, both at our laboratory and at other diagnostic laboratories utilizing the VADDS laboratory information management system. PARTICIPANTS: Dr. David H. Zeman (PI); Dr. Tanya D. Graham, Mr. Rajesh Parmar, and Mr. Jon Greseth (key personnel); and the diagnosticians and technicians of the South Dakota Animal Disease Research & Diagnostic Laboratory. TARGET AUDIENCES: The South Dakota State University (SDSU) Animal Disease Research & Diagnostic Laboratory (ADRDL) has been providing diagnostic services to the North Central Region of the U.S. since 1887. The laboratory has been continuously accredited as a full service / all species veterinary diagnostic laboratory since 1972 by the American Association of Veterinary Diagnosticians (AAVLD). The mission of the ADRDL is to provide high quality veterinary diagnostic services as a means to promptly and accurately establish cause of animal health problems. Such diagnoses aid the attending veterinarians and health officials in the surveillance, prevention, treatment, and control of animal diseases, thus benefitting the South Dakota livestock industry, animal owners, and society at large. The Veterinary & Biomedical Sciences Department at SDSU and the ADRDL are fully integrated. Numerous faculty and staff have split appointments in the areas of diagnostic service, research, Extension, and teaching. The ADRDL is authorized to conduct PCR, serology, and immunohistochemistry (IHC) testing via the National Animal Health Laboratory Network (NAHLN) training and proficiency programs. The NAHLN grant funds provided this past year have allowed us to increase South Dakota's diagnostic capabilities and capacity in preparation for responding to animal disease outbreaks, especially diseases with significant public health or economic consequences. It has also provided for continued training opportunities relative to NAHLN development and NAHLN led initiatives. PROJECT MODIFICATIONS: Not relevant to this project.

Impacts
The goals of this project are to enhance the capability and capacity of State and University animal disease diagnostic laboratories to perform/provide 1) surveillance for routine and exotic/foreign animal diseases, 2) early detection of foreign animal diseases, 3) a rapid response to disease outbreaks, and 4) the appropriate and timely recovery from an animal agriculture catastrophe. The presence of a network of diagnostic laboratories means that facilities, personnel, and expertise are available nationwide, no matter where a disease outbreak occurs. The mission statement of the National Animal Health Laboratory Network (NAHLN) includes the following: provide timely, accurate and consistent services nationwide with a focus on exotic, zoonotic, and emerging diseases of livestock and non-livestock species; provide laboratory data to meet epidemiological and disease reporting needs, and to maintain the laboratory network capacity and capability in order to respond to foreign animal disease outbreaks or other adverse animal health events. This includes both routine testing for endemic diseases (e.g. influenza viruses in swine and poultry) as well as active surveillance for foreign animal diseases such as Foot and Mouth Disease, Classical Swine Fever, Exotic Newcastle Disease. Surveillance for diseases of public health interest such as the prion-associated diseases (Bovine Spongiform Encephalopathy in cattle, Chronic Wasting Disease of deer and elk, and Scrapie disease in sheep) and novel (aka: pandemic) H1N1 influenza virus in swine are also an important facet of the animal disease surveillance provided by the NAHLN.

Publications

• No publications reported this period

Progress 09/01/09 to 08/31/10

Outputs
OUTPUTS: During the 2009-2010 hunting season the SDSU ADRDL joined with the South Dakota Game Fish and Parks Department and Wildlife Services to test >650 wild birds for Avian Influenza Virus infection, using the PCR test (Avian Influenza matrix screen) followed, in the case of a positive matrix test result, by H5 and H7 PCR tests. The SDSU ADRDL also tests wild bird samples from other states as a member of the NAHLN. The lab continues to work with the state meat inspection programs of ND and SD to test all farm raised cervids such as deer and elk for Chronic Wasting Disease (CWD) via the immunohistochemistry (IHC) test. ADRDL also performs Scrapie IHC testing for the various regulatory programs designed to aid in the eradication of Scrapie. In addition to the regulatory programs, ADRDL has joined with South Dakota Game Fish and Parks Department during the 2009-2010 deer and elk hunting seasons, to perform surveillance testing for CWD utilizing the BioRad ELISA with confirmatory CWD IHC testing. To date, the lab has completed the following surveillance tests for this fiscal year (July 1 2009 thru June 15, 2010): Exotic Newcastle Disease-98 PCR tests; Avian Influenza Matrix-1396 PCR tests; Avian Influenza-792 Serology tests; Avian Influenza H5/H7-508 PCR tests; Swine Influenza Virus-1516 Serology tests; Swine Influenza Virus-89 PCR tests; Swine Influenza Virus N1-2 PCR tests; Foot & Mouth Disease-98 PCR tests; Classical Swine Fever-330 PCR tests; Chronic Wasting Disease-840 ELISA tests; Scrapie-955 IHC tests. With regard to training, IT / NAHLN development training has included attendance by the ADRDL IT specialist, the ADRDL quality manager, and faculty at the following meetings: ABSL3Ag and ABSL3 - Special Species (ABSL3+). Atlanta, Georgia. January 24, 2010. (Tanya Graham) AAVLD Laboratory Safety Committee Symposium. San Diego, California. October 11, 2009. (Tanya Graham; Rajesh Parmar) American Association of Veterinary Laboratory Diagnosticians 52th Annual Conference. San Diego, California. Oct. 10-12, 2009. (Rajesh Parmar; David Zeman; Jon Greseth; Regg Neiger; Tanya Graham) AAVLD Accreditation & Quality Assurance Symposium. San Diego, California. October 8, 2009. (Rajesh Parmar, Tanya Graham) To date we have successfully sent test messages to the NAHLN repository computer and have received test messages from NAHLN. We also recently completed upgrades of the VADDS table structure to handle necessary NAHLN data fields. Refinements such as this remove barriers relative to VADDS interfacing with the NAHLN, both at our laboratory and at other diagnostic laboratories utilizing the VADDS laboratory information management system. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: The South Dakota State University (SDSU) Animal Disease Research & Diagnostic Laboratory (ADRDL) has been providing diagnostic services to the North Central Region of the U.S. since 1887. The laboratory has been continuously accredited as a full service / all species veterinary diagnostic laboratory since 1972 by the American Association of Veterinary Diagnosticians (AAVLD). The mission of the ADRDL is to provide high quality veterinary diagnostic services as a means to promptly and accurately establish cause of animal health problems. Such diagnoses aid the attending veterinarians and health officials in the surveillance, prevention, treatment, and control of animal diseases, thus benefitting the South Dakota livestock industry, animal owners, and society at large. The Veterinary & Biomedical Sciences Department at SDSU and the ADRDL are fully integrated. Numerous faculty and staff have split appointments in the areas of diagnostic service, research, Extension, and teaching. The ADRDL is authorized to conduct PCR, serology, and immunohistochemistry (IHC) testing via the National Animal Health Laboratory Network (NAHLN) training and proficiency programs. The NAHLN grant funds provided this past year have allowed us to increase South Dakota's diagnostic capabilities and capacity in preparation for responding to animal disease outbreaks, especially diseases with significant public health or economic consequences. It has also provided for continued training opportunities relative to NAHLN development and NAHLN led initiatives. PROJECT MODIFICATIONS: Not relevant to this project.

Impacts
The goals of this project are to enhance the capability and capacity of State and University animal disease diagnostic laboratories to perform/provide 1) surveillance for routine and exotic/foreign animal diseases, 2) early detection of foreign animal diseases, 3) a rapid response to disease outbreaks, and 4) the appropriate and timely recovery from an animal agriculture catastrophe. The presence of a network of diagnostic laboratories means that facilities, personnel, and expertise are available nationwide, no matter where a disease outbreak occurs. The mission statement of the National Animal Health Laboratory Network (NAHLN) includes the following: provide timely, accurate and consistent services nationwide with a focus on exotic, zoonotic, and emerging diseases of livestock and non-livestock species; provide laboratory data to meet epidemiological and disease reporting needs, and to maintain the laboratory network capacity and capability in order to respond to foreign animal disease outbreaks or other adverse animal health events. This includes both routine testing for endemic diseases (e.g. Swine Influenza Virus) as well as active surveillance for foreign animal diseases such as Foot and Mouth Disease, Classical Swine Fever, Exotic Newcastle Disease, and Influenza viruses in swine and poultry. Surveillance for diseases of public health interest such as the prion-associated diseases (Bovine Spongiform Encephalopathy in cattle, Chronic Wasting Disease of deer and elk, and Scrapie disease in sheep) and novel (aka: pandemic) H1N1 influenza virus in swine are also an important facet of the animal disease surveillance provided by the NAHLN.

Publications

• No publications reported this period