Progress 12/15/16 to 12/14/17
Outputs Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Graduate student research training. The student will present a poster at the Plant and Animal Genome Conference in 2018 How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?In the next year, we will focus on RNA analyses of chloroplast transcriptsof cold-tolerant and susceptible cucumber lines. We will determine the level and postions of RNA editing to establish if any differences occur after editing. We will also determine if the candidate SNP in the chloroplast DNA is edited.
Impacts What was accomplished under these goals?
Chloroplast DNAs were purified from cold-tolerant and susceptible lines of cucumber. The cold-tolerant line has a single nucleotide polymorphism (SNP) in one of the subunits of atpase, which results in an amino acid change. This specific SNP is the candidate for cold tolerance.
Publications
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Progress 12/15/15 to 12/14/16
Outputs Target Audience:Cucumber breeders and producers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?A graduate student was hired and she started on September 1, 2016. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?We plan to complete next-generation sequencing of chloroplast DNAs from cold-tolerant and susceptible doubled-haploid lines, and evaluate for sublimons that may be associated with cold tolerance.
Impacts What was accomplished under these goals?
Doubled haploid (DH) cucumber lines were produced and evaluated under cold stress. The DH from the heirloom cultivar 'Chipper' suffered significantly less damage by cold compared to other DH lines from cultivars 'Straight 8' and '9930'. Hybrids were made between the Chipper DH with the cold-susceptible DHs and evaluated for cold tolerance. The hybrids with Chipper as the maternal parent performed significantly better than the reciprocal hybrids. Chloroplast DNA was isolated from DHs of Chipper and Straight 8 for next-generation sequencing.
Publications
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