Source: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY submitted to
PROTECTING POLLINATORS WITH ECONOMICALLY FEASIBLE AND ENVIRONMENTALLY SOUND ORNAMENTAL HORTICULTURE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
NEW
Funding Source
OTHER GRANTS
Reporting Frequency
Annual
Accession No.
1010197
Grant No.
2016-51181-25399
Project No.
NJ27961
Proposal No.
2016-04929
Multistate No.
(N/A)
Program Code
SCRI
Project Start Date
Sep 1, 2016
Project End Date
Aug 31, 2018
Grant Year
2016
Project Director
Palmer, C. L.
Recipient Organization
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
3 RUTGERS PLZA
NEW BRUNSWICK,NJ 08901-8559
Performing Department
IR-4
Non Technical Summary
Protecting pollinators has risen to a high level of public interest and is impacting decision making at many levels from individual consumer to the federal government. This research project "Protecting Pollinators with Economically Feasible and Environmentally Sound Ornamental Horticulture" will provide crucial, science-based information for this decision making and provide opportunities for the ornamental horticulture industry to contribute to improved pollinator health by growing plants using the best production practices, thereby increasing pollinator forage quality and quantity in rural and urban landscapes.First, this research project will study the attractiveness of ornamental horticulture plants for honey bees, bumble bees and other native pollinators by identifying pollinators visiting newly established test garden plots and by identifying pollen collected by bees deposited at their hives. Second, this research project will investigate the concentration of systemic pesticides in pollen and nectar to determine whether residues exceed safe levels with current ornamental horticulture production practices. Third, this research project will compare current and alternative insect management strategies for economics (cost to apply), efficacy (ability to manage pests effectively), and toxicology (impact on mammals, birds, fish, and the environment). Fourth, this research project will examine people's perceptions and purchasing habits for pollinator attractive plants by surveying US homeowners for their likelihood for purchasing plants with different prices, production practices and vocabulary for pollinator attractiveness. Fifth, using the information from the above research experiments, we will design best management practices for growers and landscape professionals to deal with pest problems while protecting pollinators. Overall, these studies will improve pollinator health by identifying pollinator attractive plants currently available in the marketplace and the pollinators which visit them, documenting the actual risk to pollinators from current and alternative ornamental horticulture production practices, crafting guidelines for pollinator education displays at garden centers and public gardens, and developing recommendations for growers and landscape professionals for effective pest management while protecting pollinators. This project will protect pollinators and improve the sustainability and profitability of the ornamental horticulture and beekeeping industries.
Animal Health Component
0%
Research Effort Categories
Basic
30%
Applied
60%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2162110113010%
2162120113010%
2162123113010%
2112199115025%
6036299301015%
2063099113030%
Knowledge Area
211 - Insects, Mites, and Other Arthropods Affecting Plants; 216 - Integrated Pest Management Systems; 603 - Market Economics; 206 - Basic Plant Biology;

Subject Of Investigation
2120 - Herbaceous perennials and decorative greens; 2110 - Ornamental trees and shrubs; 3099 - Bees, honey, and other pollinators, general; 6299 - Marketing, general/other; 2123 - Bedding/garden plants; 2199 - Ornamentals and turf, general/other;

Field Of Science
1130 - Entomology and acarology; 3010 - Economics; 1150 - Toxicology;
Goals / Objectives
Goal: This project will aid in restoring and enhancing pollinator habitat by providing growers and landscape managers the knowledge to address pests while producing high quality plants for pollinator forage.Objective 1) Identify nursery and greenhouse plants attractive to pollinatorsThis will be accomplished by cataloguing previous published information, screening the top 15 - 20 selling plant species and cultivars regionally for pollinator visitation, comparing pollinator visitations to cultivars within species where variation is expected, and identifying pollen collected from bumble bee hives placed in landscapes with rich and diverse flowering plants.Objective 2) Determine pollen, nectar and floral insecticide residues from current ornamental horticulture production practicesThis team will apply perennial and annual crops according to insecticide label directions and collect pollen, nectar, and whole flowers depending on species. Plant species with a single blooming period (determinate) will have applications made at specific intervals prior to bloom with samples collected at bloom. Plant species with continuous blooming (indeterminate) will have single applications made prior to crop finish date with samples collected over time. Collected samples will be analyzed using standard procedures for multi-pesticide residue screens.Objective 3) Compare economics, efficacy, and toxicology of alternative management practicesThis team will propose several alternative pest management programs to minimize exposure of pollinators to systemic insecticides during bloom. These alternative practices will be assessed for economic feasibility and efficacy. The mammalian, avian, and eco-toxicology profiles of alternative products will be compared. In addition, we will determine the factors influencing growers' production practice adoption by surveying current production practices and future alternative practices and how adoption might affect production costs.Objective 4) Assess impact of pollinator labelling on consumer purchasing habits while identifying consumer perceptions and purchasing barriersThis will be accomplished using computer-based surveys of US homeowners to determine the impact of pollinator labelling for plants on purchasing and willingness to pay premiums. These surveys will also identify factors affecting consumer perceptions of and their current activities to improve pollinator health and identify barriers to purchasing pollinator-attractive plants. The computer-based survey will be validated with eye tracking experiments to assess visual attention for garden center and public garden displays.Objective 5) Develop best management practices to protect pollinatorsUtilizing the knowledge gathered from above-listed research activities, best management practices will be developed to inform and modify grower production and landscape maintenance practices. Recommendations may include different pest management practices for indeterminate and determinate flowering crops and distinct options for pest management programs for highly, moderately, minimally attractive to pollinators, and crops not providing bee forage.
Project Methods
Objective 1) Identify nursery and greenhouse plants attractive to pollinatorsIdentification of plant species and cultivars attractive to pollinators will be achieved by 1) selecting top selling regionally and nationally important plants and cultivars in species expected to have variability in pollinator attractiveness, 2) establishing replicate open field blocks containing randomized single species plot in six locations across the US, 3) monitoring pollinator visitation during peak bloom by recording target bee species for 5 minutes and netting during an additional 5 minute period, and 4) identifying pollen collected by bumble bees. Pollinator observations will be restricted to the top 10 most easily identifiable generalist species. For all sites, visits of honey bees and bumble bees will be recorded. Sweat bees, leafcutter bees, mason bees and mining bees and other genera will also be monitored. Pollen from bumble bee hives will be collected weekly from May through Sept. and identified using traditional palynology with light microscopy and published visual keys and high throughput genetic meta-barcoding.Results analysis/interpretation: Preference for plant species will be tested by a simple ranks-based index. Bee abundance, richness, and diversity using the Shannon index will be compared using a mixed model analysis of covariance. General linear models will be used to test whether the floral abundance affected the total abundance of bees. Depending on the results, degrees of attraction will be established ranging from not attractive to highly attractive. Degree of attraction will be used as rough indicator of potential bee exposure from examined ornamental horticulture plants.Objective 2) Determine pollen, nectar and floral insecticide residues from current ornamental horticulture production practicesSystemic insecticides will be applied to perennial and annual crops according to label directions. Subsequently, pollen, nectar, and whole flowers will be collected from treated plants during the blooming period; samples collected will depend on plant species. Species with a single blooming period (determinate) will have single applications made at specific intervals prior to bloom with samples collected at bloom. Plant species with continuous blooming (indeterminate) will have single applications made prior to crop finish date with samples collected over time. Pesticide analyses of collected samples will be conducted using standard procedures for multi-pesticide residue screens. The samples will be extracted via a modified QuEChERS protocol followed by instrumental (HPLC/MS/MS) or immunological (ELISA) analysis.Results analysis/interpretation: Regression analyses of residue data will identify the time to reach peak concentrations and subsequent half-lives for residues in floral samples for each insecticide and type of plant tested. This will allow modeling for the use of these insecticides to reach target residue levels that should not be injurious to pollinators. Residue concentrations will be compared to acute and chronic levels of concern to aid in data interpretation. For example, 25 ppb is the EPA level of concern for imidacloprid. Residues below this level are not expected to harm pollinators.Objective 3) Compare economics, efficacy, and toxicology of alternative management practicesProduction costs will be collected from greenhouse/nursery growers (via mail and electronic questionnaires) The survey will include questions on costs if neonicotinoid-based treatments were restricted or banned, such as convenience of application, labor/time saving, worker safety, and relative efficacy of neonicotinoids when compared to alternatives. The survey data will be used to calculate differences in production costs considering 1) substitution to alternative insecticides, 2) changes in efficacy of alternative products, 3) changes in labor costs, and 4) changes is costs associated with addressing new worker safety requirements.We will compile efficacy information for important target insect pests considering both soil drench and foliar application methods. This information will be gathered from previously published and non-published efficacy reports. For products lacking adequate efficacy information, replicated, controlled trials will be conducted on infested pollinator-attractive plants.We will compile ecotoxicological profiles for alternative products to neonicotinoids including conventional insecticides, biopesticides and minimum-risk pesticides.Results analysis/interpretation: Economic feasibility will be analyzed using an enterprise budgeting-based economic model. Collected data from both efficacy and phytotoxicity trials will be subjected to statistical analysis using usual multiple comparison procedures.Objective 4) Assess impact of pollinator labelling on consumer purchasing habits while identifying consumer perceptions and purchasing barriersComputer-based surveys will be combined with eye tracking/visual attention analysis to collect data from consumers. First, online choice experiments and surveys will be administered to US homeowners. The experiments will consist of alternative product choices where participants select which option they would purchase. The alternative products will be comprised of pre-determined attributes and attribute levels. Participants also have a chance to select a 'neither' option. Second, a smaller study will be conducted using eye tracking software to determine consumer gaze behavior for plant sales displays. This data will be analyzed in relation to consumers' willingness to pay and socio-demographic variables.Objective 5) Develop best management practices to protect pollinatorsWe will develop a matrix that contains a list of pollinator-attractive plants listed by family or genera, and categorize them by level of attractiveness, flowering times (determinate and indeterminate), potential pest pressure from sucking insects (most susceptible to systemic insecticides) and pest management practices, including effective alternative strategies.Results analysis/interpretation: A thorough review of results obtained in Objectives 1-4 by all project personnel and key stakeholders will assist in decision-making and final recommendations to protect pollinators.EFFORTSA website will be developed to post results, extension materials, pest management plans, fact sheets and slides for extension educators, news releases, and other materials such as a quarterly newsletter and webcasts. Pollinator attractiveness will be catalogued into a searchable public database. Residue results will be shared with registrants and regulators to inform risk assessments and label development Information from the economic survey, efficacy studies, and ecotoxicology profiles will be distilled into a data matrix, posted to the web. Scientific manuscripts and technical extension bulletins will be prepared and published. Extension presentations and print/online publications will be prepared and distributed. Guidance for consumer point of purchase materials and public garden displays will be developed.EVALUATIONWe will track unique visits to the database and anticipate that there will be increased traffic over time. Plug producers and growers will shift offerings to plant species and cultivars identified as pollinator attractive via visitation counts to plots and identification of bumble bee-collected pollen. Depending on residue analysis of pollen and nectar, use directions for registered products may be updated to mitigate pollinator risk; we will monitor any potential label modifications. We will assess grower adoption of new best management practices. In addition to specific metrics for each objective to track milestone achievements and success, a global project rubric was developed to evaluate overall project success covering research objectives, outreach objectives, administrative reporting, and project team communications.

Progress 09/01/16 to 08/31/17

Outputs
Target Audience:The target audiences reached during the first year of this project have been primarily growers of flowering ornamental horticulture crops, consumers, extension personnel, and researchers within entomology. Changes/Problems:For Obj 1, some of the perennial species selected did not establish well and needed to be replenished during the growing season. Some of the perennials produced fewer flowers than anticipated during the first year and did not attract as many pollinators based on expected visitation patterns for more established plantings in anecdotal or published reports. We anticipate that flowering will be more abundant during the second year of data collection. For Obj 2, in the NJ location, Phytophthora root rot contributed to a reduction of rhododendron stand despite relying heavily on preventative fungicide treatments. This location will restart this study in spring 2018, but results will be delayed by one year in comparison to the companion plantings at other locations. No other major setbacks have occurred. What opportunities for training and professional development has the project provided?The opportunities for professional development have been primarily for the young professionals participating in the research activities and corrolary project managment tasks. Graduate students (Emily Ericson, Bernadetter Mach) and post doctoral candidate (Doug Sponsler) had the opportunity to present their ongoing research at grower and landscaper meetings and at the annual meeting of the Entomological Society of America. Yu-Han Lan, a Taiwanese national pursuing post-Master professional development, has been developing project mangement skills by assisting in the oversight of the diverse research avenues. Ms. Lan has also been developing science communication skills by creating content for the Protecting Bees project website. Amy Abate, an undergraduate work-study student, has been refining her online data search skills to locate plant information to populate the pollinator attractiveness database. We have also provided her assignments that draw on agriculture economics, her major area of study, to give her first-hand knowledge of the value and impact macro and micro economics can have on decision-making outcomes. How have the results been disseminated to communities of interest?Primarily, our team members have been communicating results from research funded prior to the start of this grant. They have written scientific and trade journal articles and have given presentations to grower/landscaper association meetings and to scientific society meetings. They have also granted interviews to the media about practical ways consumers can protect pollinators on their own properties. In addition, our researcher and stakeholder team members contributed to the Horticultural Research Institute's BMP which was posted on their website along with print copies being distributed at trade shows including Cultivate. Our team has developed a website https://protectingbees.njaes.rutgers.edu to highlight the research objectives, relay status of research activities, and summarize results as well as serve as an online robust pollinator attractiveness database for consumers and the scientific community. What do you plan to do during the next reporting period to accomplish the goals?Our planned activities for each objective are listed below. Obj 1. Pollinator Attractiveness We will continue to collect data on pollinators to perennials established in the field plots during 2017. The annuals will be replenished with new plantings. We will continue to collect pollen from honey bees returning to their hives and develop DNA barcoding for plant family and genera detection along with identifying pollen using morphology. Obj 2. Insecticide Residues We will be establishing and/or initiating residue work on the following plant species: Geraldton wax flower (nectar), dahlia (pollen), snapdragon (nectar), annual salvia (nectar), perenial salvia (nectar), and red hot poker (nectar). We will continue to refine the analytical methods to improve recovery of cyantraniliprolle. Obj 3. Comparisons of Alternatives We will continue to compile management tool options for aphids, fungus gnats, mites and thrips. We will then compile efficacy data for mealybugs, scale, and whiteflies. At the same time, we will compile environmental toxicology data. We will administer the producer survey and enterprise budget spreadsheet, analyze data, and begin to write manuscripts and present findings. Obj. 4. Pollinator-Related Consumer Perceptions and Purchasing Habits We will administer the eye tracking and online surveys for consumers, analyze data, and begin to write manuscripts and present findings. Obj. 5. Best Management Practices As the attractiveness data and residue data develop, we will refine recommendations and begin to draft new outreach materials.

Impacts
What was accomplished under these goals? Protecting pollinators has risen to a high level of public interest and is impacting decision making at many levels from individual consumer to the federal government. This research project will provide crucial, science-based information for this decision making and provide opportunities for the ornamental horticulture industry to contribute to improved pollinator health by growing plants using the best production practices, thereby increasing pollinator forage quality and quantity in rural and urban landscapes. During the first year, we have established test garden plots of common annuals and perennials and collected/counted the visiting pollinators. We have begun studies on the amount of systemic insecticides found in pollen and nectar with plots of rhododendron and sunflower and have established plots for geraltdon wax flower, salvia, and snapdragon. We have started compiling the available efficacy and toxicology information for alternative treatment options and have developed the grower survey to understand the economic and social impacts related to neonicotinoid use or lack thereof. We also developed the consumer online and eye tracking survey tools to assess consumer willingness to pay and preferences related to grower production practices. We contributed to the Horticultural Research Institute's Best Management Practices document and wrote more than 10 articles and gave more than 40 presentations. Ultimately, these activities will improve pollinator health and improve the sustainability and profitability of the ornamental horticulture and beekeeping industries. OUTCOMES Obj 1. Pollinator Attractiveness Major activities: We established six garden plots with crops from the top 25 annuals and perennials according to NASS Census of Horticulture, 2014 in southern and northern CA (Bethke, Casey), CT (Stoner), MI (Smitley), PA (Grozinger, Patch) and SC (Chong). We counted the number of pollinators visiting the plants during morning and afternoon timed periods and collected pollinators with sweep net captures for later identification. In KY (Potter), we continued to identify pollinators collected in a previously initiated woody ornamental landscape study and analyze count data. In CT and PA, we have collected pollen from returning honey bee drones for future identification of visited plant species through morphology and DNA bar-coding. Data collected/Summary of results: In KY, more than 16,000 pollinator specimens were collected from 75 species of flowering woody landscape plants spread among ~375 sites. All pollinators have been identified to genus and selected families to species. Outcomes: It is commonly thought that native plants are better to foster bee diversity than introduced plants or highly developed cultivars. However, we discovered when comparing visitation rates and bee diversity that native and non-native trees and shrubs were similar. Non-native plants in KY extend the overall bloom period particularly in fall when many natives are not blooming. We now know that non-native species can provide forage for pollinators. Obj 2. Insecticide Residues Major activities: Because sample sizes may be less than the optimal 1 ml nectar or 1 mg pollen, Dr. Thayaril refined and validated analytical methods to extract and quantify the 5 applied active ingredients and their relevant metabolites by examining solvent composition, multiple column chemistries, and injection volumes. During spring 2017, we started rhododendron insecticide residue experiments in CT (Cowles), KY (Potter), MI (Smitley), NJ (Palmer). We collected baseline nectar and pollen samples during flowering but before applications. In CA (Bethke), CT (Cowles), and SC (Chong), we initiated sunflower pollen studies during summer 2017. We assessed the ability for several annual plants to produce nectar to determine best model plants and the number of plants needed. During summer 2017, bumble bee colonies were placed into screen tents for three weeks with seven popular annuals previously drenched with imidacloprid (MI). After the exposure period, colonies were placed near a naturalized landscape. Bumble bees in each colony were counted semimonthly and whole flowers were analyzed for imidacloprid. Data collected/Summary of results: The final optimized procedure (extraction, cleanup, LC-MS/MS) indicated that the lowest amount of neonicotinoid that could be detected from a 100 µl sample volume was 2.5 ppb which is ~2 times more sensitive than the previously reported method for low sample volume using the QuEChERs method. Collected rhododendron baseline and sunflower samples have not yet been analyzed. Bumble bee colonies declined steadily throughout the summer with those exposed to imidacloprid averaging 25 to 20% fewer workers than non-exposed colonies. Five of the seven annuals contained imidacloprid in flower tissues, but pollen and nectar were not analyzed specifically for residues. Outcomes: The improvement in analytical detection with very small sample sizes will enable our team to analyze samples that might otherwise be discarded or pooled with samples from other reps or collections. It was highly unexpected that two of the seven annuals contained no detectable imidacloprid in whole flowers. This highlights the diversity of systemic insecticide uptake, internal plant movement, and decline within the many plant species grown in the green industry, clearly demonstrating that we need to be careful not to over-generalize or over-simplify future guidelines for growers. Obj 3. Comparisons of Alternatives Major activities: To determine gaps in understanding, we have begun comparing available efficacy data to manage aphids, fungus gnats, mites, and thrips with neonicotinoids and alternatives including biopesticides. We developed a preliminary list of products as alternatives for the toxicological and economic comparisons. We developed an online survey tool to query producers on their current production practices to manage pests and created enterprise budget spreadsheets for more detailed economic analysis of individual operations. Data collected/Summary of results: N/A Outcomes: N/A Obj. 4. Pollinator-Related Consumer Perceptions and Purchasing Habits Major activities: Dr. Khachatryan developed two survey tools to assess consumers' perceptions of grower production practices using choice analysis. Both incorporate the same questions with visuals and product attributes (pollinator labelling, pricing, etc). The difference is that the in-person study utilizes eye-tracking software to determine consumer attention to elements of the display whereas the online survey does not. The in-person study (~200 responses) is being conducted fall 2017, and the online survey (1,000 responses) will be conducted winter 2018. Data collected/Summary of results: N/A Outcomes: N/A Obj. 5. Best Management Practices Major activities: Key members of our research and stakeholder team developed the first BMP for the ornamental horticulture industry based on the limited knowledge available in winter 2017. This was published as Horticultural Research Institute's "Best Management Practices (BMPs) for Bee Health in the Horticultural Industry, Version 1.0". Data collected/Summary of results: N/A. Outcomes: Cross-industry partnerships to develop BPMs are a successful process to transform newly generated knowledge into relevant grower and landscape management practices.

Publications

  • Type: Websites Status: Published Year Published: 2017 Citation: https://protectingbees.njaes.rutgers.edu/
  • Type: Other Status: Other Year Published: 2017 Citation: Smitley, D. and A. Melathopolous. 2017. "Greenhouse and nursery practices for protecting pollinators". Podcast for the state of Washington, March 2017.
  • Type: Other Status: Other Year Published: 2017 Citation: Smitley, D. 2017. "The role of nurseries and landscapers in protecting and enhancing pollinators". Michigan Nursery and Landscape Assocation Pollinator Conference, Okemos, Michigan, March 31, 2017. (125 attendees)
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Mach BM, Bondarenko S, Potter DA. 2017. Uptake and dissipation of neonicotinoid residues in nectar and foliage of systemically-treated woody landscape plants. Environmental Toxicology and Chemistry. 2017 Oct 28. doi: 10.1002/etc.4021.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Redmond CT, Potter DA. 2017. Chlorantraniliprole: Reduced-risk insecticide for controlling insect pests of woody ornamentals with low hazard to bees. Arboriculture and Urban Forestry. 2017. 43(6): 242-256.
  • Type: Other Status: Published Year Published: 2017 Citation: Mach BM. McNamara TD, Potter DA. 2017. Creating pollinator-friendly landscapes. Greenhouse Product News. January; 2427. http://www.gpnmag.com/article/creating-pollinator-friendly-landscapes/
  • Type: Other Status: Published Year Published: 2017 Citation: Mach, B.M. and D.A. Potter. Plants bees like best. Growwise.org. Horticultural Research Institute. February 2017.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Rich Cowles*, Cristi Palmer, James Bethke, Juang-Horng Chong, Brian Eitzer, Dan Potter, Dave Smitley, Nishanth Thayaril. 2017. "Systemic insecticide risk assessment for pollinators in ornamental horticulture crops". Presentation at SemiAnnual American Chemical Society Meeting. Aug 2017.
  • Type: Other Status: Other Year Published: 2017 Citation: Smitley, D. 2017. "The pollinators pallate: a healthy mix of annuals and perennials". Cultivate17, Columbus, Ohio, July 17, 2017. (85 attendees)
  • Type: Other Status: Awaiting Publication Year Published: 2017 Citation: Smitley, D. 2017. "A Balancing Act: Saving Trees from Exotic Pests While Promoting Pollinators and Beneficial Insects". Tree Care Industry Association Magazine. In press.
  • Type: Other Status: Other Year Published: 2016 Citation: Stoner, K. 2016. The Push for Pollinators  Needs for Plants and Seeds. CAES Valley Laboratory Tour, September 15, 2016. (30 attendees)
  • Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Stoner, K. 2016. Increasing Pollinators by Increasing Plant Diversity. Annual CT NOFA Organic Land Care Meeting in Southington, CT. December 9, 2016. (135 attendees)
  • Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Stoner, K. 2017. An Act Concerning Pollinator Health: State Efforts to Protect Pollinators in Connecticut. Annual Eastern Branch Meeting of the Entomological Society of America, March 20, 2017.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Stoner, K. 2017. Pollinators in the Garden. Milford Garden Club, April 11, 2017. (67 attendees)
  • Type: Other Status: Other Year Published: 2017 Citation: Stoner, K., B Eitzer, R Cowles, A Nurse. 2017. Detecting Pesticides in Plant Pollen. Annual open house of CAES, Plant Science Day. (1200 attendees).
  • Type: Other Status: Published Year Published: 2017 Citation: Mach BM. McNamara TD, Potter DA. 2017. "Creating pollinator-friendly landscapes. Greenhouse Product News. January; 2427. http://www.gpnmag.com/article/creating-pollinator-friendly-landscapes/